Abstract SNACC-79

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Limited value of Laser-Doppler Flowmetry for Intraluminal Middle Cerebral Artery Occlusion in Wistar rats

Taninishi H, Jung J, Sheng H, Izutsu M, Wang Z, Warner D
Duke University Medical Center, Durham, NC, USA

Background; Laser Doppler flowmetry (LDF) is widely used for detecting the change in cerebral blood flow (CBF) during intraluminal middle cerebral artery occlusion (MCAO), but no report has examined its efficacy in standardizing outcome. We examined MCAO histologic and behavioral outcome as a function of LDF measurement.
Method; Male Wistar rats were subjected to 90 min MCAO by 4 surgeons having various levels of experience in performing MCAO. LDF was measured in all rats during ischemia. By random assignment, LDF values were (Assisted) or were not (Blinded) made available to the surgeon during MCAO (n = 12 – 17). Neurological and histological outcomes were measured 7 days post-MCAO.
Results; %LDF change was not different between the two groups for pooled across surgeons (P = 0.12) (Figure 1A) and individual surgeons (P = 0.31 to 0.69) (Figure 1B). There was no difference in neurological scores (Assisted vs. Blinded = 14 ± 6 vs. 13 ± 7, P = 0.61) (Figure 2A) or cerebral infarct volume (162 ± 63 mm3 vs. 143 ± 86 mm3, P = 0.24) (Figure 2B). Only in one novice, LDF use increased infarct volume (145 ± 28 mm3 vs. 98 ± 61 mm3, P = 0.03). Overall, LDF use decreased infarct volume coefficient of variation by 35%, with absence of homogeneity of variance between Blinded and Assisted groups (P = 0.02) (Figure 3).
Conclusions; LDF measurement did not alter neurological or histologic outcome. LDF did decrease coefficient of variation regardless of surgical experience, which might allow use of fewer animals to test hypotheses.

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